Efficient In Vitro Regeneration of Chrysanthemum (Chrysanthemum morifolium Ramat.) Through Nodal Explant Culture

An efficient in vitro plant regeneration protocol was developed for Chrysanthemum morifolium, an ornamental plant. Nodal segments were used as explants in the present experiment. The explants were cultured onto Murashige & Skoog’s (MS) nutrient medium containing plant growth regulators (cytokinins or auxins) with various combinations and concentrations for the study. Maximum percentage (93.33%), best shoot induction per culture (21.73 ± 1.19) and highest shoot length (6.43±0.31) were achieved on MS medium supplemented with 1.0 mg/l BAP and 0.1 mg/l IAA. With repeated subculture on the same medium increase the number of shoots per culture. For rooting, in vitro raised well developed shoots were cultured onto half strength MS medium supplemented with different concentrations of IBA, IAA and NAA. The isolated shoots rooted well (98%) within 4 weeks on half-strength MS fortified with 0.2 mg/l IBA, where average number of roots per shoot was 12.27 ± 0.53. After transplantation, regenerated plantlets developed in natural condition variation.